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oscc cell lines fadu  (Procell Inc)
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SLC8A1-AS1 is downregulated in oral squamous cell carcinoma. A Venn diagram illustrating lncRNAs derived from TCGA data and GeneCards. B Expression levels of lncRNAs in human oral keratinocytes (NHOK) and <t>OSCC</t> cell lines were assessed using qRT-PCR. C SLC8A1-AS1 expression in HNSC based on TCGA data. D SLC8A1-AS1 expression in OSCC based on TCGA data. * P < 0.05 vs. pc-NC group
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SLC8A1-AS1 is downregulated in oral squamous cell carcinoma. A Venn diagram illustrating lncRNAs derived from TCGA data and GeneCards. B Expression levels of lncRNAs in human oral keratinocytes (NHOK) and OSCC cell lines were assessed using qRT-PCR. C SLC8A1-AS1 expression in HNSC based on TCGA data. D SLC8A1-AS1 expression in OSCC based on TCGA data. * P < 0.05 vs. pc-NC group

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: SLC8A1-AS1 is downregulated in oral squamous cell carcinoma. A Venn diagram illustrating lncRNAs derived from TCGA data and GeneCards. B Expression levels of lncRNAs in human oral keratinocytes (NHOK) and OSCC cell lines were assessed using qRT-PCR. C SLC8A1-AS1 expression in HNSC based on TCGA data. D SLC8A1-AS1 expression in OSCC based on TCGA data. * P < 0.05 vs. pc-NC group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Derivative Assay, Expressing, Quantitative RT-PCR

Overexpression of SLC8A1-AS1 reduces proliferation and migration of OSCC cells. A Expression levels of SLC8A1-AS1 in OSCC cells after SLC8A1-AS1 overexpression was assessed using qRT-PCR. B Proliferation of OSCC cells was assessed using the CCK-8 assay. C Proliferation of OSCC cells was evaluated using the EdU assay (Scale bar = 50 μm). D Invasion of OSCC cells were measured using the Transwell assay (Scale bar = 100 μm). E Migration of OSCC cells was analyzed through a wound healing assay. F Gene and protein levels of Ki67, MCM3, E-cad, and N-cad in OSCC cells were assessed using the qRT-PCR and western blot. * P < 0.05 vs. pc-NC group

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: Overexpression of SLC8A1-AS1 reduces proliferation and migration of OSCC cells. A Expression levels of SLC8A1-AS1 in OSCC cells after SLC8A1-AS1 overexpression was assessed using qRT-PCR. B Proliferation of OSCC cells was assessed using the CCK-8 assay. C Proliferation of OSCC cells was evaluated using the EdU assay (Scale bar = 50 μm). D Invasion of OSCC cells were measured using the Transwell assay (Scale bar = 100 μm). E Migration of OSCC cells was analyzed through a wound healing assay. F Gene and protein levels of Ki67, MCM3, E-cad, and N-cad in OSCC cells were assessed using the qRT-PCR and western blot. * P < 0.05 vs. pc-NC group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Over Expression, Migration, Expressing, Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Transwell Assay, Wound Healing Assay, Western Blot

SLC8A1-AS1 functions as a sponge for hsa-miR-1305. A Venn diagram illustrating the targets genes of SLC8A1-AS1. B Expression levels of miR-1305 in HNSC based on TCGA data. C Expression levels of miR-1305 in OSCC based on TCGA data. D Expression levels of miR-1305 in NHOK and OSCC cell lines were assessed using qRT-PCR. * P < 0.05 vs. NHOK group. E Predicted binding sites between SLC8A1-AS1 and miR-1305 are determined by lncRNASNP V3. F Expression levels of miR-1305 following transfection with miR-1305 mimics were assessed using the qRT-PCR. * P < 0.05 vs. mimics NC group. G The interaction between SLC8A1-AS1 and miR-1305 was validated using a dual-luciferase reporter gene assay. * P < 0.05 vs. mimics NC group. H The interaction between SLC8A1-AS1 and miR-1305 was confirmed through an RNA pull-down assay. * P < 0.05 vs. Bio-NC group. I Expression levels of miR-1305 in OSCC cells following overexpression of SLC8A1-AS1. * P < 0.05 vs. pc-NC group

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: SLC8A1-AS1 functions as a sponge for hsa-miR-1305. A Venn diagram illustrating the targets genes of SLC8A1-AS1. B Expression levels of miR-1305 in HNSC based on TCGA data. C Expression levels of miR-1305 in OSCC based on TCGA data. D Expression levels of miR-1305 in NHOK and OSCC cell lines were assessed using qRT-PCR. * P < 0.05 vs. NHOK group. E Predicted binding sites between SLC8A1-AS1 and miR-1305 are determined by lncRNASNP V3. F Expression levels of miR-1305 following transfection with miR-1305 mimics were assessed using the qRT-PCR. * P < 0.05 vs. mimics NC group. G The interaction between SLC8A1-AS1 and miR-1305 was validated using a dual-luciferase reporter gene assay. * P < 0.05 vs. mimics NC group. H The interaction between SLC8A1-AS1 and miR-1305 was confirmed through an RNA pull-down assay. * P < 0.05 vs. Bio-NC group. I Expression levels of miR-1305 in OSCC cells following overexpression of SLC8A1-AS1. * P < 0.05 vs. pc-NC group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Transfection, Luciferase, Reporter Gene Assay, Pull Down Assay, Over Expression

ESRRG is the target protein of miR-1305. A A Venn diagram depicting the target genes of miR-1305. B The expression levels of ESRRG in HNSC based on TCGA data. C The expression levels of ESRRG in OSCC based on TCGA data. D The mRNA expression levels of ESRRG in NHOK and OSCC cell lines. * P < 0.05 vs. NHOK group. E The binding sites between miR-1305 and ESRRG predicted by TargetScan 8.0. F The target relationship between miR-1305 and ESRRG was validated using a dual-luciferase reporter gene assay. * P < 0.05 vs. mimics NC group. G The interaction between ESRRG and miR-1305 was confirmed through an RNA pull-down assay. * P < 0.05 vs. Bio-NC group. H The expression levels of ESRRG in OSCC cells after miR-1305 overexpression. * P < 0.05 vs. pc-NC group. H The expression levels of ESRRG in OSCC cells after SLC8A1-AS1 overexpression. * P < 0.05 vs. mimics NC group

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: ESRRG is the target protein of miR-1305. A A Venn diagram depicting the target genes of miR-1305. B The expression levels of ESRRG in HNSC based on TCGA data. C The expression levels of ESRRG in OSCC based on TCGA data. D The mRNA expression levels of ESRRG in NHOK and OSCC cell lines. * P < 0.05 vs. NHOK group. E The binding sites between miR-1305 and ESRRG predicted by TargetScan 8.0. F The target relationship between miR-1305 and ESRRG was validated using a dual-luciferase reporter gene assay. * P < 0.05 vs. mimics NC group. G The interaction between ESRRG and miR-1305 was confirmed through an RNA pull-down assay. * P < 0.05 vs. Bio-NC group. H The expression levels of ESRRG in OSCC cells after miR-1305 overexpression. * P < 0.05 vs. pc-NC group. H The expression levels of ESRRG in OSCC cells after SLC8A1-AS1 overexpression. * P < 0.05 vs. mimics NC group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Expressing, Binding Assay, Luciferase, Reporter Gene Assay, Pull Down Assay, Over Expression

SLC8A1-AS1 inhibits the proliferation and invasion of OSCC cells by regulating the expression of miR-1305 and ESRRG. A The expression levels of ESRRG in OSCC cells were assessed using the qRT-PCR and western blot. B The proliferation of OSCC cells was assessed using the CCK-8 assay. C The proliferation of OSCC cells was evaluated using the EdU assay (Scale bar = 50 μm). D The invasion of OSCC cells were measured using Transwell assay (Scale bar = 100 μm). * P < 0.05 vs. pc-NC group, # P < 0.05 vs. SLC8A1-AS1 group, & P < 0.05 vs.SLC8A1-AS1 + miR-1305 mimics group

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: SLC8A1-AS1 inhibits the proliferation and invasion of OSCC cells by regulating the expression of miR-1305 and ESRRG. A The expression levels of ESRRG in OSCC cells were assessed using the qRT-PCR and western blot. B The proliferation of OSCC cells was assessed using the CCK-8 assay. C The proliferation of OSCC cells was evaluated using the EdU assay (Scale bar = 50 μm). D The invasion of OSCC cells were measured using Transwell assay (Scale bar = 100 μm). * P < 0.05 vs. pc-NC group, # P < 0.05 vs. SLC8A1-AS1 group, & P < 0.05 vs.SLC8A1-AS1 + miR-1305 mimics group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, EdU Assay, Transwell Assay

SLC8A1-AS1 inhibits the migration of OSCC cells by regulating the expression of miR-1305 and ESRRG. A The migration of OSCC cells was assessed using the wound healing assay. B The expression levels of Ki67, MCM2, N-cad, and E-cad in OSCC cells were analyzed using the qRT-PCR and western blot. * P < 0.05 vs. pc-NC group, # P < 0.05 vs. SLC8A1-AS1 group, & P < 0.05 vs.SLC8A1-AS1 + miR-1305 mimics group

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: SLC8A1-AS1 inhibits the migration of OSCC cells by regulating the expression of miR-1305 and ESRRG. A The migration of OSCC cells was assessed using the wound healing assay. B The expression levels of Ki67, MCM2, N-cad, and E-cad in OSCC cells were analyzed using the qRT-PCR and western blot. * P < 0.05 vs. pc-NC group, # P < 0.05 vs. SLC8A1-AS1 group, & P < 0.05 vs.SLC8A1-AS1 + miR-1305 mimics group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Migration, Expressing, Wound Healing Assay, Quantitative RT-PCR, Western Blot